"Dissecting" a "Cell" and Examining its Components
Purpose- What are positive indicator tests for proteins, carbohydrates, and fats? Do parts of an egg test positive for protein, carbohydrates, and/or fat?
Materials-
Materials-
Procedure Part I
1. Place an uncooked egg into a beaker of white vinegar. Label and cover it with plastic wrap. Leave it for 24 hrs to 48 hrs. While the shell dissolves, proceed to Part II.
2.After 24 hrs to 48 hrs, the shell should have dissolved in the vinegar (acetic acid). Gently pick up the egg with a slotted spoon and rinse it to remove the vinegar. A chemical reaction has happened here. What chemicals were involved as reactants and products.
3.Feel the outer membrane of the cell. The outer membrane, which is different from the membrane around the yolk, provides a good model of a cell membrane . They are thin and flexible They are permeable to some substances. To verify that water can enter and leave the cell easily, place the egg in a beaker of 5% Na Cl solution for 24 hrs. Describe the appearance of the egg after 24 hrs. What may have caused the change in appearance.
4.Gently rinse off the egg again with water. Then place the egg in a beaker of distilled water for 24 hrs. Describe the appearance of the egg after 24 hrs. What the change in appearance?
5. To retrieve the egg cell, gently slice open the eggs membranes, and let the egg white drip through the slots of the spoon into a 100-mL. Beaker. Get rid of all egg white.
6. Place the egg yolk into another beaker, and set aside the egg membranes.
2.After 24 hrs to 48 hrs, the shell should have dissolved in the vinegar (acetic acid). Gently pick up the egg with a slotted spoon and rinse it to remove the vinegar. A chemical reaction has happened here. What chemicals were involved as reactants and products.
3.Feel the outer membrane of the cell. The outer membrane, which is different from the membrane around the yolk, provides a good model of a cell membrane . They are thin and flexible They are permeable to some substances. To verify that water can enter and leave the cell easily, place the egg in a beaker of 5% Na Cl solution for 24 hrs. Describe the appearance of the egg after 24 hrs. What may have caused the change in appearance.
4.Gently rinse off the egg again with water. Then place the egg in a beaker of distilled water for 24 hrs. Describe the appearance of the egg after 24 hrs. What the change in appearance?
5. To retrieve the egg cell, gently slice open the eggs membranes, and let the egg white drip through the slots of the spoon into a 100-mL. Beaker. Get rid of all egg white.
6. Place the egg yolk into another beaker, and set aside the egg membranes.
Procedure Part II
1.Glucose- put in a test mix 2 mL of 2% glucose solution with 2 mL Benedict solutions. Heat for 2 min in a boiling hot water bath and record all changes in color and record the time it takes for the color changes.
2. Water mix 2 mL of Deionized water with Benedict Solution and heat it up. Record all changes.
3. To test for starch mix 2 mL of Starch with 0.25 of Iodine/ to test the water switch the starch with it(record all changes to)
4. Protein Indicator Standard Test: To test for protein, place 2 mL of gelatin in a test tube and add 0.5 mL of 10% NaOH, swirl to mix. Add 0.25 mL of 5% biuret solution and mix well for thirty seconds./To test for water, place 2 mL of deionized water instead of protein
5. Lipid indicator test put oil/water mix Sudan IV
2. Water mix 2 mL of Deionized water with Benedict Solution and heat it up. Record all changes.
3. To test for starch mix 2 mL of Starch with 0.25 of Iodine/ to test the water switch the starch with it(record all changes to)
4. Protein Indicator Standard Test: To test for protein, place 2 mL of gelatin in a test tube and add 0.5 mL of 10% NaOH, swirl to mix. Add 0.25 mL of 5% biuret solution and mix well for thirty seconds./To test for water, place 2 mL of deionized water instead of protein
5. Lipid indicator test put oil/water mix Sudan IV
Procedure Part III
Do the same tests but this time substitute the bases with the the egg. Also record results and color changes.
DATA/RESULTS
Part 1
CaCO3 + CH3COOH > H2CO3 + H2O + Co2
DAY 1
We insert the egg into a cup of vinegar
DAY 2
The shell was dissolved and we inserted the egg into a cup of NaCl.
Day 3
The egg shrank and we then inserted it into a cup of deionized water and the egg grew back to size.
Day 4
Cut the egg open
Part 1
CaCO3 + CH3COOH > H2CO3 + H2O + Co2
DAY 1
We insert the egg into a cup of vinegar
DAY 2
The shell was dissolved and we inserted the egg into a cup of NaCl.
Day 3
The egg shrank and we then inserted it into a cup of deionized water and the egg grew back to size.
Day 4
Cut the egg open
Data and Results
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Reflection
In this lab I really enjoyed experimenting with all the different chemicals too see their chemical reactions with each other. It was also interesting to see when a failure would come out aka when the reaction was negative. Another thing I liked doing is seeing the egg expand and contract when you put it in salt and fresh water. I feel like Logan and I worked well together but I think we could've worked a little bit faster. If we did this lab again I think that we should have split up the sections between us and shared our data and results.
Data Anylasis
I think some factors that could lead to errors are the fact that humans will make mistakes. So human error was the main mistake factor. Some examples of error would be not using the right amount of liquid. Another application would be mixing all the chemicals together and seeing what happens. The data is above